Effect of ozone therapy on redox status in experimentally induced arthritis

M.Nabil. Mawsouf, Maha.M. El-Sawalhi, Hebatalla A. Darwish, Amira A. Shaheen, Gregorio Martínez-Sánchez, Lamberto Re


Controlled ozone administration has been shown to promote an adaptation to oxidative stress by increasing endogenous antioxidant systems. In the present study, the effects of O2/O3 administration either prophylactically or therapeutically on the alterations of oxidant status in adjuvant-induced arthritis in rats have been studied. Seven groups of rats were used: 1) normal control group; 2) control arthritic group (21 days); 3) prophylactic ozone group: arthritic rats received fifteen intra-rectal applications of O2/O3 at 0.5, 0.7 and 1 mg/kg b.w. in a 5-6 mL volume starting one day before adjuvant inoculation and continued as five applications/week over 21 days; 4) oxygen group: received oxygen (vehicle of ozone) in a similar schedule to group 3; 5) control arthritic group (24 days); 6) therapeutic-ozone group: arthritic rats received 10 intra-rectal applications of O2/O3 at 0.5, 0.7 and 1 mg/kg b. w. in a 5-6 mL volume daily for 10 days starting fourteen days after adjuvant inoculation; 7) oxygen-treated group: received oxygen in a similar schedule of group 6. The effect of O2/O3 administration was assessed by measuring: blood glutathione (GSH), erythrocyte glutathione peroxidase and catalase activities, serum levels of protein thiols (PrSH), malondialdehyde (MDA) and nitrite/nitrate (NO2/NO3), as well as serum ceruloplasmin activity (CP). The present study showed that adjuvant-induced arthritis in rats caused a significant (p<0.05) reduction in blood GSH, serum PrSH levels and erythrocyte antioxidant enzyme activities accompanied by a significant (p<0.05) increase in serum levels of MDA, NO2/NO3 and CP activity. Ozone administration either prophylactically or therapeutically normalize blood GSH, serum PrSH and MDA levels and restored erythrocyte antioxidant enzyme activities. However ozone did not significantly (p>0.05) modify serum NO2/NO3 level in induced rat but significant (p<0.05) increase CP activity. So it could be concluded that O2/O3 oxidative preconditioning / postconditioning effectively modulate the antioxidant/oxidant balance associated with adjuvant arthritis model in rats.

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